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Efficient in vivo editing of OTC-deficient patient-derived

Lay summary. The ability to efficiently and safely correct disease-causing mutations remains the holy grail of gene therapy. Herein, we demonstrate, for the first time, efficient in vivo correction of a patient-specific disease-causing mutation in the OTC gene in primary human hepatocytes, using therapeutically relevant vector doses.

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See detailed specifications and technical data for Schwing BP 4000 HDR-D manufactured in 1999 ... However, incomplete data and mistakes might occur.

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In humans, mean frequency differences for tracts of at least 20 bp (means per kb 1.51 in hot spots and 0.80 in cold regions, p = 0.022) and 24 bp (means per kb 0.92 in hot spots and 0.34 in cold regions p = 0.036) were statistically significant for PPTs with one mismatch per 10 bp allowed.

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